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integrin β3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc integrin β3
    Integrin β3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+%CE%B23+integrin/Integrin+beta3+XP+Rabbit+mAb/pmc13012068-115-24-27
    Average 95 stars, based on 189 article reviews
    integrin β3 - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    other:

    Article Title: The formin DAAM1 regulates the deubiquitinase activity of USP10 and integrin homeostasis.
    Article Snippet: Samples were then diluted in 4X Sample Buffer (2% SDS, 10% glycerol, 1% β-mercaptoethanol, 12.5 mM EDTA, 0.02% bromophenol blue in 50 mM Tris (pH 6.8)), boiled at 95 ◦C for 5 min, and loaded in pre-cast, 10% polyacrylamide gels (ThermoFisher; Waltham, MA).

    Article Title: Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via Ferroptosis
    Article Snippet: Protein extracts (15 μg) were subjected to electrophoresis on either 10% or 12% sodium dodecyl-sulfate-polyacrylamide gels and subsequently transferred to polyvinylidene difluoride membranes (Merck Millipore, Burlington, MA, USA).

    Incubation:

    Article Title: CRISPR-mediated activation of autism gene Itgb3 restores cortical network excitability via mGluR5 signaling
    Article Snippet: .. After incubation with primary rabbit anti-β3 integrin (1:200; cat. no. 4702, Cell Signaling); rabbit anti-mGluR1 (1:1,000; cat. no. 191 002, Synaptic Systems); rabbit anti-mGluR5 (1:200; cat. no. AB5675, Millipore), mouse anti-GluA2 (1:500; cat. no. MAB397, Millipore); rabbit anti-GluA2/3 (1:200; cat. no. 07-598, Millipore); rabbit anti-GluA1 (1:500; cat. no. AB1504, Millipore); rabbit anti-GluA1 phosphoSer831 (1:500; cat. no. AB5847, Millipore); rabbit anti-GluA1 phosphoSer845 (1:500; cat. no. AB5849, Millipore); rabbit anti-Homer1a (1:1,000; cat. no. 160 013, Synaptic Systems); or rabbit anti-β-tubulin III (1:1,000; cat. no. T2200, Sigma) antibodies, membranes were incubated with secondary HRP-conjugated goat anti-rabbit antibody (1: 5,000; cat. no. 31460, Thermo Fisher Scientific) or goat anti-mouse antibody (1: 5,000; cat. no. 31430, Thermo Fisher Scientific), and immunocomplexes were detected with the chemiluminescent substrate (cat. no. RPN2106, ECL Prime Western Blotting System, GE Healthcare). .. We acquired chemiluminescent signals using a ChemiDoc imaging system (Biorad) and quantified immunoreactive bands using ImageJ ( http://rsb.info.nih.gov/ij ).

    Article Title: Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via Ferroptosis.
    Article Snippet: Protein extracts (15 μg) were subjected to electrophoresis on either 10% or 12% sodium dodecylsulfate-polyacrylamide gels and subsequently transferred to polyvinylidene difluoride membranes (Merck Millipore, Burlington, MA, USA). .. The membranes were blocked with 2% milk in phosphate-buffered saline (PBS) and incubated with the following anti-human antibodies: rabbit anti-β3-integrin (1:1000; 13166, Cell Signaling Technology [CST], Danvers, MA, USA), rabbit anti-xCT (1:1000; 126915, CST), rabbit anti-GPX4 (1:1000; ab125066, Abcam), rabbit anti-p-4EBP1 (1:1000; 1344345, CST), rabbit anti-4EBP1 (1:1000; 9644, CST), rabbit anti-p-70S6K (1:1000; 9202S, CST), rabbit anti-p-RPS6 (1:1000; 2215S, CST), anti-ppaxillin (1:1000; 69363, CST), and rabbit anti-Cyclin D1 (1:1000, ab134175, Abcam). .. Actin (1:5000; MA5-15739, Thermo Fisher Scientific), Tubulin (1:1000; 2128, CST), and HSP90 (1:1000; MA1-10379, Thermo Scientific) were used as the protein loading control.

    Western Blot:

    Article Title: CRISPR-mediated activation of autism gene Itgb3 restores cortical network excitability via mGluR5 signaling
    Article Snippet: .. After incubation with primary rabbit anti-β3 integrin (1:200; cat. no. 4702, Cell Signaling); rabbit anti-mGluR1 (1:1,000; cat. no. 191 002, Synaptic Systems); rabbit anti-mGluR5 (1:200; cat. no. AB5675, Millipore), mouse anti-GluA2 (1:500; cat. no. MAB397, Millipore); rabbit anti-GluA2/3 (1:200; cat. no. 07-598, Millipore); rabbit anti-GluA1 (1:500; cat. no. AB1504, Millipore); rabbit anti-GluA1 phosphoSer831 (1:500; cat. no. AB5847, Millipore); rabbit anti-GluA1 phosphoSer845 (1:500; cat. no. AB5849, Millipore); rabbit anti-Homer1a (1:1,000; cat. no. 160 013, Synaptic Systems); or rabbit anti-β-tubulin III (1:1,000; cat. no. T2200, Sigma) antibodies, membranes were incubated with secondary HRP-conjugated goat anti-rabbit antibody (1: 5,000; cat. no. 31460, Thermo Fisher Scientific) or goat anti-mouse antibody (1: 5,000; cat. no. 31430, Thermo Fisher Scientific), and immunocomplexes were detected with the chemiluminescent substrate (cat. no. RPN2106, ECL Prime Western Blotting System, GE Healthcare). .. We acquired chemiluminescent signals using a ChemiDoc imaging system (Biorad) and quantified immunoreactive bands using ImageJ ( http://rsb.info.nih.gov/ij ).

    Article Title: Dysregulated cortical excitability and tau phosphorylation in a β3 integrin mouse model of autism
    Article Snippet: As described in detail in the , we used the following primary antibodies for immunohistochemistry: rabbit anti-SK2 (1:200; Cat. No. SK2-Rb-Af500; Frontier Institute), rabbit monoclonal anti-β3 integrin (1:100; Cat. No. 13166, Cell Signaling), chicken anti-GFP antibody (1:1000; Cat. No.AB13970, Abcam) and rabbit anti-Er81 antibody (1:200; Cat. No. Ab81086, Abcam). .. As described in detail in the , we used the following antibodies for western blotting: rabbit anti-β3 integrin (1:200; Cat. No. 4702, Cell Signaling), rabbit anti-SK2 (1:200; Cat. No. SK2-Rb-Af500, Frontier Institute), mouse anti-phospho Tau (AT8, Ser202/Thr205; 1:2000; Cat. No. MN1020, ThermoFisher), mouse anti-Tau (TAU-5; 1:1000; Cat. No. AHB0042, ThermoFisher) and rabbit anti-β-tubulin III (1:1000; Cat. No. T2200, Sigma). .. As described in detail in the , we used the following antibodies for immunoprecipitation: hamster anti-β3 integrin (Cat. No. 553343; BD Pharmingen), rabbit anti-SK2 (Cat. No. SK2-Rb-Af500, Frontier Institute), hamster IgG (Cat. No. PA5-33219 ThermoFisher) and rabbit anti-RFP (Cat. No. 600-401-379, Rockland).

    Article Title: Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin
    Article Snippet: .. Mouse anti-Cdc42, Mouse anti-GM130, Mouse anti-BrdU (for IHC), Mouse anti-ROCKII (BD Biosciences), Goat anti-DCT TRP2, Mouse anti-T-MLC MRCL3/MRCL2/MYL9 (E-4), Rabbit-antiWAVE2 (Santa Cruz), Mouse anti-Rac1 (Cytoskeleton), Mouse anti-vinculin (hVIN1, Sigma), Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19), Rabbit anti-Phospho Myosin Light Chain 2 (Ser19) for immunofluorescence (Cell Signalling Technology), Rabbit anti-phospho-paxillin, Rabbit antiGAPDH, Rabbit anti-α4 integrin for Western blots, Rabbit anti-β3 integrin, Rabbit anti-α6 integrin (Cell Signalling), Rabbit anti-α4 integrin (D2E1, Cell Signalling Technology, for immunofluorescence) rabbit anti-Ki67 (SP6, Neomarkers), Mouse anti-BrdU (Dako), Rabbit anti-p34-Arc, Rat anti-β1 integrin (Millipore), Mouse-anti GAPDH (Ambion) Rabbit anti-α-Tubulin, Rat anti-tyrosinated tubulin, mouse anti-FMNL2 antibody (Abcam) Mouse anti-Talin (8D4, Sigma), Sheep anti-MITF (kind gift of Claudia Wellbrock, Manchester University, UK). .. Monoclonal biotinylated rabbit anti-goat IgG secondary antibody was obtained from Dako.

    Saline:

    Article Title: Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via Ferroptosis.
    Article Snippet: Protein extracts (15 μg) were subjected to electrophoresis on either 10% or 12% sodium dodecylsulfate-polyacrylamide gels and subsequently transferred to polyvinylidene difluoride membranes (Merck Millipore, Burlington, MA, USA). .. The membranes were blocked with 2% milk in phosphate-buffered saline (PBS) and incubated with the following anti-human antibodies: rabbit anti-β3-integrin (1:1000; 13166, Cell Signaling Technology [CST], Danvers, MA, USA), rabbit anti-xCT (1:1000; 126915, CST), rabbit anti-GPX4 (1:1000; ab125066, Abcam), rabbit anti-p-4EBP1 (1:1000; 1344345, CST), rabbit anti-4EBP1 (1:1000; 9644, CST), rabbit anti-p-70S6K (1:1000; 9202S, CST), rabbit anti-p-RPS6 (1:1000; 2215S, CST), anti-ppaxillin (1:1000; 69363, CST), and rabbit anti-Cyclin D1 (1:1000, ab134175, Abcam). .. Actin (1:5000; MA5-15739, Thermo Fisher Scientific), Tubulin (1:1000; 2128, CST), and HSP90 (1:1000; MA1-10379, Thermo Scientific) were used as the protein loading control.

    Cell-Signaling:

    Article Title: Dysregulated cortical excitability and tau phosphorylation in a β3 integrin mouse model of autism
    Article Snippet: As described in detail in the , we used the following primary antibodies for immunohistochemistry: rabbit anti-SK2 (1:200; Cat. No. SK2-Rb-Af500; Frontier Institute), rabbit monoclonal anti-β3 integrin (1:100; Cat. No. 13166, Cell Signaling), chicken anti-GFP antibody (1:1000; Cat. No.AB13970, Abcam) and rabbit anti-Er81 antibody (1:200; Cat. No. Ab81086, Abcam). .. As described in detail in the , we used the following antibodies for western blotting: rabbit anti-β3 integrin (1:200; Cat. No. 4702, Cell Signaling), rabbit anti-SK2 (1:200; Cat. No. SK2-Rb-Af500, Frontier Institute), mouse anti-phospho Tau (AT8, Ser202/Thr205; 1:2000; Cat. No. MN1020, ThermoFisher), mouse anti-Tau (TAU-5; 1:1000; Cat. No. AHB0042, ThermoFisher) and rabbit anti-β-tubulin III (1:1000; Cat. No. T2200, Sigma). .. As described in detail in the , we used the following antibodies for immunoprecipitation: hamster anti-β3 integrin (Cat. No. 553343; BD Pharmingen), rabbit anti-SK2 (Cat. No. SK2-Rb-Af500, Frontier Institute), hamster IgG (Cat. No. PA5-33219 ThermoFisher) and rabbit anti-RFP (Cat. No. 600-401-379, Rockland).

    Immunohistochemistry:

    Article Title: Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin
    Article Snippet: .. Mouse anti-Cdc42, Mouse anti-GM130, Mouse anti-BrdU (for IHC), Mouse anti-ROCKII (BD Biosciences), Goat anti-DCT TRP2, Mouse anti-T-MLC MRCL3/MRCL2/MYL9 (E-4), Rabbit-antiWAVE2 (Santa Cruz), Mouse anti-Rac1 (Cytoskeleton), Mouse anti-vinculin (hVIN1, Sigma), Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19), Rabbit anti-Phospho Myosin Light Chain 2 (Ser19) for immunofluorescence (Cell Signalling Technology), Rabbit anti-phospho-paxillin, Rabbit antiGAPDH, Rabbit anti-α4 integrin for Western blots, Rabbit anti-β3 integrin, Rabbit anti-α6 integrin (Cell Signalling), Rabbit anti-α4 integrin (D2E1, Cell Signalling Technology, for immunofluorescence) rabbit anti-Ki67 (SP6, Neomarkers), Mouse anti-BrdU (Dako), Rabbit anti-p34-Arc, Rat anti-β1 integrin (Millipore), Mouse-anti GAPDH (Ambion) Rabbit anti-α-Tubulin, Rat anti-tyrosinated tubulin, mouse anti-FMNL2 antibody (Abcam) Mouse anti-Talin (8D4, Sigma), Sheep anti-MITF (kind gift of Claudia Wellbrock, Manchester University, UK). .. Monoclonal biotinylated rabbit anti-goat IgG secondary antibody was obtained from Dako.

    Immunofluorescence:

    Article Title: Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin
    Article Snippet: .. Mouse anti-Cdc42, Mouse anti-GM130, Mouse anti-BrdU (for IHC), Mouse anti-ROCKII (BD Biosciences), Goat anti-DCT TRP2, Mouse anti-T-MLC MRCL3/MRCL2/MYL9 (E-4), Rabbit-antiWAVE2 (Santa Cruz), Mouse anti-Rac1 (Cytoskeleton), Mouse anti-vinculin (hVIN1, Sigma), Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19), Rabbit anti-Phospho Myosin Light Chain 2 (Ser19) for immunofluorescence (Cell Signalling Technology), Rabbit anti-phospho-paxillin, Rabbit antiGAPDH, Rabbit anti-α4 integrin for Western blots, Rabbit anti-β3 integrin, Rabbit anti-α6 integrin (Cell Signalling), Rabbit anti-α4 integrin (D2E1, Cell Signalling Technology, for immunofluorescence) rabbit anti-Ki67 (SP6, Neomarkers), Mouse anti-BrdU (Dako), Rabbit anti-p34-Arc, Rat anti-β1 integrin (Millipore), Mouse-anti GAPDH (Ambion) Rabbit anti-α-Tubulin, Rat anti-tyrosinated tubulin, mouse anti-FMNL2 antibody (Abcam) Mouse anti-Talin (8D4, Sigma), Sheep anti-MITF (kind gift of Claudia Wellbrock, Manchester University, UK). .. Monoclonal biotinylated rabbit anti-goat IgG secondary antibody was obtained from Dako.



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